Determination of 3'-azido-3'-deoxythymidine, 2',3'-dideoxycytidine, 3'-fluoro-3'-deoxythymidine and 2',3'-dideoxyinosine in biological samples by high-performance liquid chromatography.
J Chromatogr
; 534: 101-7, 1990 Dec 14.
Article
en En
| MEDLINE
| ID: mdl-1965595
ABSTRACT
A simple and fast high-performance liquid chromatographic assay for the determination of 3'-azido-3'-deoxythymidine (AZT), 2',3'-dideoxycytidine (ddC), 3'-fluoro-3'-deoxythymidine (FT) and 2',3'-dideoxy-inosine (ddI) in complex biological matrices is described. The method allows rapid nucleoside determination using a phenyl column within extra- as well as intracellular media without further sample pretreatment and extraction procedures. The lower limit of detection is ca. 0.05 micrograms/ml for each nucleoside, and the separation can easily be optimized for AZT, ddC, FT and ddI by variation of the methanolic part of the mobile phase and the detector wavelengths.
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Banco de datos:
MEDLINE
Asunto principal:
Antivirales
/
Didesoxinucleósidos
/
Cromatografía Líquida de Alta Presión
Límite:
Animals
/
Humans
Idioma:
En
Año:
1990
Tipo del documento:
Article