Reprogramming human fibroblasts using HIV-1 TAT recombinant proteins OCT4, SOX2, KLF4 and c-MYC.
Mol Biol Rep
; 37(4): 2117-24, 2010 Apr.
Article
en En
| MEDLINE
| ID: mdl-19669668
ABSTRACT
It has been shown that human and murine fibroblasts can be reprogrammed by ectopic expression of transcription factors using viral vectors. For the purpose of human therapeutic applications, the integration of viral transgenes into the genome is unlikely to be accepted. We therefore produced recombinant transcription factor proteins in E. coli (OCT4, SOX2, c-MYC and KLF4) carrying the cell penetrating TAT domain from HIV1. The purified proteins were able to enter into mammalian cells when added to tissue culture medium but appeared not to translocate to the nucleus. Further investigation indicated that most of the protein was tied up in the endosomes and was unavailable for reprogramming. Once this problem has been solved it seems likely that protein reprogramming will be the method of choice for clinical applications.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
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Proteínas Recombinantes de Fusión
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VIH-1
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Reprogramación Celular
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Productos del Gen tat del Virus de la Inmunodeficiencia Humana
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Fibroblastos
Límite:
Humans
Idioma:
En
Año:
2010
Tipo del documento:
Article