[Simultaneous determination of four flavonoids in Malus prunifolia from Shanxi province by RP-HPLC].
Zhongguo Zhong Yao Za Zhi
; 34(17): 2217-9, 2009 Sep.
Article
en Zh
| MEDLINE
| ID: mdl-19943489
ABSTRACT
OBJECTIVE:
To develop an HPLC method for simultaneous determination of rutin, hyperoside, quercetin and kaempferol in Malus prunifolia from Shanxi province in China.METHOD:
The separation was performed on a Hypersil C18 column (4.6 mm x 250 mm, 5 microm), using a gradient elution with methanol-water containing 0.2% phosphoric acid as the mobile phase. The flow rate was 1.0 mL x min(-1), the detection wavelength was 360 nm and the temperature of column was 25 degrees T.RESULT:
The linear ranges of rutin, hyperoside, quercetin and kaempferol were 1.87-46.67 mg x L(-1), 6.40-160.0 mg x L(-1), 3.33-83.33 mg x L(-1), 0.80-20.00 mg x L(-1), respectively. The average recoveries (n=6) of the four constituents were 99.2% (RSD 2.9%), 98.2% (RSD 1.9%), 97.4% (RSD 2.3%), 97.2% (RSD 1.3%), respectively.CONCLUSION:
The method was simple, accurate and can be used to evaluate medicinal value of Malus prunifolia.
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Banco de datos:
MEDLINE
Asunto principal:
Flavonoides
/
Medicamentos Herbarios Chinos
/
Cromatografía Líquida de Alta Presión
/
Malus
/
Cromatografía de Fase Inversa
Tipo de estudio:
Evaluation_studies
País como asunto:
Asia
Idioma:
Zh
Año:
2009
Tipo del documento:
Article