Flow minimal residual disease monitoring of candidate leukemic stem cells defined by the immunophenotype, CD34+CD38lowCD19+ in B-lineage childhood acute lymphoblastic leukemia.
Haematologica
; 95(4): 679-83, 2010 Apr.
Article
en En
| MEDLINE
| ID: mdl-19951974
ABSTRACT
Flow cytometric minimal residual disease (MRD) monitoring could become more powerful if directed towards the disease-maintaining leukemic stem cell (LSC) compartment. Using a cohort of 48 children with B-lineage acute lymphoblastic leukemia (ALL), we sought the newly proposed candidate-LSC population, CD34(+)CD38(low)CD19(+), at presentation and in end of induction bone marrow samples. We identified the candidate LSC population in 60% of diagnostic samples and its presence correlated with expression of CD38, relative to that of normal B-cell progenitors. In addition, the candidate LSC was not detectable in all MRD positive samples. The absence of the population in 40% of diagnostic and 40% of MRD positive samples does not support the use of this phenotype as a generic biomarker to track LSCs and suggests that this phenotype may be an artifact of CD38 underexpression rather than a biologically distinct LSC population. ClinicalTrials.gov Identifier NCT00222612.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Células Madre Neoplásicas
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Leucemia-Linfoma Linfoblástico de Células Precursoras B
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Neoplasia Residual
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Antígenos CD34
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Antígenos CD19
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ADP-Ribosil Ciclasa 1
Tipo de estudio:
Prognostic_studies
Límite:
Child
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Humans
Idioma:
En
Año:
2010
Tipo del documento:
Article