[Culture and identify the human umbilical vein endothelial cells and investigate the expression of tyrosine kinase-2 with immunoglobulin-like and epidermal growth factor homology domains in the cells].
Hua Xi Kou Qiang Yi Xue Za Zhi
; 27(6): 653-6, 2009 Dec.
Article
en Zh
| MEDLINE
| ID: mdl-20077904
ABSTRACT
OBJECTIVE:
To study the cultural method and identification of human umbilical vein endothelial cells (HUVECs), and investigate the expression of tyrosine kinase-2 with immunoglobulin-like and epidermal growth factor homology domains(Tie-2) in HUVECs.METHODS:
HUVECs were isolated from umbilical veins by the technique of irrigative digestion, and were cultivated in plates. The cells were identified by VIII monoclonal antibody. Tie-2 mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and SABC immunocytochemistry.RESULTS:
HUVECs could adhere to the plates completely after 24 hours, and confluence a monolayer 4-5 days later. The band of Tie-2 mRNA was obviously and the expression of Tie-2 protein was strongly positive by immunocytochemistry in HUVECs. The positive rate was over 85%.CONCLUSION:
Highly purified endothelial cells were isolated. And there were overexpression of Tie-2 in HUVECs.
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Banco de datos:
MEDLINE
Asunto principal:
TYK2 Quinasa
/
Células Endoteliales de la Vena Umbilical Humana
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
Zh
Año:
2009
Tipo del documento:
Article