Recovery of functional enzyme from amyloid fibrils.
FEBS Lett
; 584(6): 1139-42, 2010 Mar 19.
Article
en En
| MEDLINE
| ID: mdl-20132817
ABSTRACT
Amyloid deposits, which accumulate in numerous diseases, are the final stage of multi-step protein conformational-conversion and oligomerization processes. The underlying molecular mechanisms are not fully understood, and particularly little is known about the reverse reaction. Here we show that phosphoglycerate kinase amyloid fibrils can be converted back into native protein. We achieved recovery with 60% efficiency, which is comparable to the success rate of the unfolding-refolding studies, and the recovered enzyme was folded, stable and fully active. The key intermediate stages in the recovery process are fibril disassembly and unfolding followed by spontaneous protein folding.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Fosfoglicerato Quinasa
/
Amiloide
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Año:
2010
Tipo del documento:
Article