A flow cytometric assay to quantify in vivo bacterial uptake by alveolar macrophages.

Pitz, Adam M; Perry, Greg A; Jensen-Smith, Heather C; Gentry-Nielsen, Martha J

Pitz, Adam M; Perry, Greg A; Jensen-Smith, Heather C; Gentry-Nielsen, Martha J.

Pitz AM; Department of Microbiology and Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178, USA.

J Microbiol Methods ; 81(2): 194-6, 2010 May.

Article en En | MEDLINE | ID: mdl-20171249

RESUMEN

Our laboratory has developed a flow cytometric assay to quantify alveolar macrophage (Mcapital EF, Cyrillic) phagocytosis of bacteria within a live animal. Mcapital EF, Cyrillics collected by bronchoalveolar lavage from rats infected transtracheally with Syto 9-labeled bacteria are fluorescently labeled for identification and analyzed by flow cytometry to quantify their bacterial uptake.

Asunto(s)

Bacterias/inmunología; Citometría de Flujo/métodos; Macrófagos Alveolares/microbiología; Fagocitosis; Animales; Lavado Broncoalveolar; Colorantes Fluorescentes/farmacología; Pulmón/inmunología; Masculino; Compuestos Orgánicos/farmacología; Ratas; Ratas Sprague-Dawley; Coloración y Etiquetado

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Fagocitosis / Bacterias / Macrófagos Alveolares / Citometría de Flujo Tipo de estudio: Evaluation_studies Límite: Animals Idioma: En Año: 2010 Tipo del documento: Article

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