[Establishment and application of an analytical method for PINK1 gene exon copy number].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
; 27(2): 158-61, 2010 Apr.
Article
en Zh
| MEDLINE
| ID: mdl-20376796
ABSTRACT
OBJECTIVE:
To establish a method for analyzing the PTEN-induced kinase 1 gene (PINK1) exon copy number and apply it to the analysis of PINK1 gene exon copy number variation (CNV) in patients with autosomal recessive early-onset Parkinsonism (AREP).METHODS:
Real-time PCR was used to analyze the exon copy number in 22 probands with AREP from unrelated Chinese Han families and 30 healthy controls.RESULTS:
Copy numbers of exons 1-8 of the PINK1 gene were analyzed, and satisfactory reaction conditions and primers for exons of the PINK1 gene were obtained. No exon CNV in the PINK1 gene was detected in this group.CONCLUSION:
An analytical method for PINK1 gene exon copy number was established. The exon CNV in the PINK1 gene was rare in Chinese patients with AREP.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Quinasas
/
Reacción en Cadena de la Polimerasa
/
Exones
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Dosificación de Gen
Tipo de estudio:
Observational_studies
Límite:
Adolescent
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Adult
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Child
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Child, preschool
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Female
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Humans
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Male
Idioma:
Zh
Año:
2010
Tipo del documento:
Article