Protective effect of rebamipide against Helicobacter pylori-CagA-induced effects on gastric epithelial cells.
Dig Dis Sci
; 56(2): 441-8, 2011 Feb.
Article
en En
| MEDLINE
| ID: mdl-20556513
ABSTRACT
BACKGROUND:
Helicobacter pylori CagA dysregulates cell signaling pathways and leads to targeted transcriptional up-regulation of genes implicated in gastric cell injury. The aim of this study was to determine the effects of rebamipide on CagA-induced effects on gastric epithelial cells. We investigated the effects of rebamipide treatment (pre- or post-treatment before or after CagA transfection) on CagA-induced gastric cell injury.METHOD:
We evaluated the morphologic changes (hummingbird phenotype) associated with ZO-1 mislocalization by confocal microscopy, IL-8 production by ELISA, and NF-κB activation by luciferase assay in AGS gastric epithelial cells and MDCK cells.RESULTS:
Transfection of CagA into gastric epithelial cells induced morphologic changes (hummingbird phenotype), ZO-1 mislocalization, and IL-8 production in gastric epithelial cells. Pre-treatment with rebamipide inhibits CagA-induced effects on gastric epithelial cells, including morphologic changes (hummingbird phenotype) associated with ZO-1 mislocalization, IL-8 production, and NF-κB activity.CONCLUSIONS:
These results suggest that rebamipide might have a potential role in the protection of H. pylori CagA-induced effects on gastric epithelial cells.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Estómago
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Proteínas Bacterianas
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Quinolonas
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Alanina
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Células Epiteliales
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Antígenos Bacterianos
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Antioxidantes
Límite:
Animals
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Humans
Idioma:
En
Año:
2011
Tipo del documento:
Article