A gene-delivery system specific for hepatoma cells and an intracellular kinase signal based on human liver-specific bionanocapsules and signal-responsive artificial polymer.
Int J Pharm
; 396(1-2): 174-8, 2010 Aug 30.
Article
en En
| MEDLINE
| ID: mdl-20558262
ABSTRACT
Recently, our group has proposed a novel gene-regulation system responding to cAMP-dependent protein kinase (PKA) that has been applied to living cells. In this study, human liver-specific bionanocapsules (BNCs) are used as a gene-delivery system to increase transfection efficiency and to target specific cell types. BNCs can efficiently deliver a target gene to human hepatocytes and hepatoma cells in vitro or in vivo. The combination of a signal-responsive gene-delivery system with BNCs led to an increase in the transfection efficiency and selectivity for hepatoma cells. Expression from the delivered gene was identified from PKA-activated hepatoma cells (HepG2), but not from colon tumor cells (WiDr). These results show that the combination of a gene-regulation system responding to an intracellular signal with BNC can be used for the selective treatment of human hepatoma cells.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Polímeros
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Transfección
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Transducción de Señal
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Proteínas Quinasas Dependientes de AMP Cíclico
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Carcinoma Hepatocelular
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Nanocápsulas
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Neoplasias Hepáticas
Límite:
Humans
Idioma:
En
Año:
2010
Tipo del documento:
Article