Quality assurance for biogenic amines with authentic patient specimens.
Pathology
; 23(1): 11-6, 1991 Jan.
Article
en En
| MEDLINE
| ID: mdl-2062561
ABSTRACT
The measurement of biogenic amines and metabolites is essential for diagnosis and follow-up of neural crest tumors. A Quality Assurance programme involving the distribution of urine specimens obtained from patients with neural crest and related tumors was conducted by the Australian Association of Clinical Biochemists' Working Party on Biogenic Amines in 1988. Fifty laboratories participated in the programme and measured a number of analytes with a variety of methods. These included high performance liquid chromatography with electrochemical detection (HPLC-EC), spectrophotometry, fluorescence, fluorescence polarization immunoassay (Abbott TDX) and gas chromatography--mass spectroscopy (GC-MS). The results of this survey indicated that fluorimetric methods for catecholamines are unreliable as they are subject to interference particularly by Labetalol. Twice as many laboratories utilized catecholamines rather than metanephrines for detection of pheochromocytoma. 5-HIAA appears to be the analyte measured with the least accuracy, particularly with spectrophotometric methods of analysis. Several laboratories would have failed to diagnose some neural crest tumors and need to improve their performance.
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Banco de datos:
MEDLINE
Asunto principal:
Garantía de la Calidad de Atención de Salud
/
Aminas Biogénicas
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Año:
1991
Tipo del documento:
Article