Identification of the origin of replication of the eukaryote Dictyostelium discoideum nuclear plasmid Ddp2.
Plasmid
; 24(3): 208-17, 1990 Nov.
Article
en En
| MEDLINE
| ID: mdl-2077545
ABSTRACT
Ddp2 is a 5.8-kb, high-copy-number, nuclear plasmid found in the eukaryote Dictyostelium discoideum. We have identified two functional domains, a large open reading frame (Rep gene) and a 626-bp fragment containing an origin of replication (ori). The ori, when cloned into a shuttle vector, confers stable extrachromosomal replication in D. discoideum, provided that the Rep gene, which acts in trans, is integrated into the host genome. Ddp2 carries a 501-bp imperfect inverted repeat, and part of the ori overlaps with one of these repeats. The ori sequence contains two direct repeats of 49 bp comprising two 10-bp "TGTCATGACA" palindromes separated by a poly(T.A) sequence. Deletion of either 49-bp repeat abolished extrachromosomal replication.
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Banco de datos:
MEDLINE
Asunto principal:
Plásmidos
/
Núcleo Celular
/
Dictyostelium
/
Replicación del ADN
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Año:
1990
Tipo del documento:
Article