[A possible association of fatal pneumonia with mutations of pandemic influenza A/H1N1 sw1 virus in the receptor-binding site of the HA1 subunit].
Vopr Virusol
; 55(4): 4-9, 2010.
Article
en Ru
| MEDLINE
| ID: mdl-20886705
ABSTRACT
The paper gives the results of sequence analysis of 150 positive samples in real-time RT-PCR, including 47 autopsy materials from patients (including 10 pregnant women), who died from fatal pneumonia mainly in November-December 2009, in whom the lifetime etiological diagnosis had not been made and hence no early etiotropic therapy performed. 70% of the primary materials from the deceased patients were found to have pandemic influenza A(H1N1) v mutants in the lung tissue with D222G (15%), D222N (15%), D222E (2%) substitutions, as well as a mixture of mutants (38%). Nasopharyngeal lavages from 3 Chukotka deceased patients exhibited only consensus (nonmutant) D222 virus variants; there was a mixture of consensus and mutant virus variants in the trachea and a mixture of mutant ones in the lung. Preliminary data from the study of the interaction of the hemagglutinin of two strains having D222G and D222N mutations with 9 oligosaccharides imitating the variants of cell receptors for influenza A virus suggest that there is a double receptor specificity for alpha2'-3' and alpha2'-6'-sialosides with a preponderance of alpha2'-3'-specificity. Further spread of the mutants that have acquired a high virulence and preserved their capacity for the respiratory route of human infection may lead to the situation similar to that seen in the 1918-1919 pandemic. Another scenario for evolution of the virus is to preserve its receptor specificity for alpha2'-3'-sialosides and high virulence with losses of alpha2'-6' specificity and capacity for aerosol transmission, by damping the pandemic.
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Banco de datos:
MEDLINE
Asunto principal:
Neumonía Viral
/
Complicaciones Infecciosas del Embarazo
/
Brotes de Enfermedades
/
Glicoproteínas Hemaglutininas del Virus de la Influenza
/
Subunidades de Proteína
/
Gripe Humana
/
Subtipo H1N1 del Virus de la Influenza A
Tipo de estudio:
Risk_factors_studies
Límite:
Female
/
Humans
/
Male
/
Pregnancy
País como asunto:
Asia
/
Europa
Idioma:
Ru
Año:
2010
Tipo del documento:
Article