[The detection of viral antigens and ribosomal RNA in cells by using low-temperature media]. / Vyiavlenie virusnykh antigenov i ribosomal'noi RNK v kletkakh s ispol'zovaniem nizkotemperaturnykh sred.
Tsitologiia
; 32(11): 1111-3, 1990.
Article
en Ru
| MEDLINE
| ID: mdl-2093248
ABSTRACT
A post-embedding technique for immunocytochemical analysis at the ultrastructural level was used to detect and localize HIV antigens on ultrathin sections of Lowicryl-embedded HIV-infected cells. A genomic probe containing ribosomal sequences and labeled with biotin was used to hybridize rRNA molecules in sections of animal cells embedded in Lowicryl. The method presently described offers the possibility to detect rapidly and precisely ribosomal gene expression and viral proteins at the ultrastructural level.
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Banco de datos:
MEDLINE
Asunto principal:
ARN Ribosómico
/
Antígenos VIH
/
VIH-1
/
VIH-2
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
Idioma:
Ru
Año:
1990
Tipo del documento:
Article