An efficient method of directly cloning chimpanzee adenovirus as a vaccine vector.
Nat Protoc
; 5(11): 1775-1785, 2010 Nov.
Article
en En
| MEDLINE
| ID: mdl-21030953
ABSTRACT
Adenoviral vectors have shown great promise as vaccine carriers and in gene transfer to correct underlying genetic diseases. Traditionally, construction of adenoviral vectors is complex and time consuming. In this paper, we provide an improved method for efficient generation of novel adenoviral vectors by using direct cloning. We introduce a feasible and detailed protocol for the development of chimpanzee adenoviruses (Ads) as molecular clones, as well as for the generation of recombinant virus from the molecular clones. Recombinant viruses are genetically stable and induce potent immune responses in animals. Generation of new Ad molecular clones or new recombinant Ad can be achieved in 2 months or 2 weeks, respectively.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Adenovirus de los Simios
/
Clonación Molecular
/
Vectores Genéticos
Idioma:
En
Año:
2010
Tipo del documento:
Article