Stand-alone self-powered integrated microfluidic blood analysis system (SIMBAS).
Lab Chip
; 11(5): 845-50, 2011 Mar 07.
Article
en En
| MEDLINE
| ID: mdl-21152509
ABSTRACT
We present a self-powered integrated microfluidic blood analysis system (SIMBAS) that does not require any external connections, tethers, or tubing to deliver and analyze a raw whole-blood sample. SIMBAS only requires the user to place a 5 µL droplet of whole-blood at the inlet port of the device, whereupon the stand-alone SIMBAS performs on-chip removal of red and white cells, without external valving or pumping mechanisms, followed by analyte detection in platelet-containing plasma. Five complete biotin-streptavidin sample-to-answer assays are performed in 10 min; the limit of detection is 1.5 pM. Red and white blood cells are removed by trapping them in an integral trench structure. Simulations and experimental data show 99.9% to 100% blood cell retention in the passive structure. Powered by pre-evacuation of its PDMS substrate, SIMBAS' guiding design principle is the integration of the minimal number of components without sacrificing effectiveness in performing rapid complete bioassays, a critical step towards point-of-care molecular diagnostics.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Integración de Sistemas
/
Técnicas Analíticas Microfluídicas
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Pruebas Hematológicas
Límite:
Humans
Idioma:
En
Año:
2011
Tipo del documento:
Article