Mutation of isoleucine 705 of the oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae affects lanosterol's C/D-ring cyclization and 17α/ß-exocyclic side chain stereochemistry.
Org Biomol Chem
; 9(4): 1092-7, 2011 Feb 21.
Article
en En
| MEDLINE
| ID: mdl-21157613
ABSTRACT
Site-saturated substitution in Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase at Ile705 position produced three chair-boat-chair (C-B-C) truncated tricyclic compounds, two 17α-exocyclic protosteryl intermediates, two protosteryl C-17 truncated rearranged intermediates and the normal biosynthetic product, lanosterol. These results indicated the importance of the Ile705 residue in affecting lanosterol's C/D ring stabilization including 6-6-5 tricyclic and protosteryl C-17 cations and 17α/ß-exocyclic side chain stereochemistry.
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Banco de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
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Transferasas Intramoleculares
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Lanosterol
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Mutación
Idioma:
En
Año:
2011
Tipo del documento:
Article