Large-scale production, concentration and purification of Rous Sarcoma Virus in tissue culture.
Folia Biol (Praha)
; 24(4): 225-32, 1978.
Article
en En
| MEDLINE
| ID: mdl-212330
ABSTRACT
A method is described for long-term standard production of purified virus from cultures of PR-RSV-C-transformed chicken cells. The mean yields were 4.91 mg of purified virus per liter of culture medium. Cells were grown in long-term cultures for 26 weeks. A combination of polyethylene glycol 6 000 precipitation and zonal centrifugation has been found satisfactory for virus concentration and purification.
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Banco de datos:
MEDLINE
Asunto principal:
Virus del Sarcoma Aviar
Límite:
Animals
Idioma:
En
Año:
1978
Tipo del documento:
Article