Purinergic signaling is required for fluid shear stress-induced NF-κB translocation in osteoblasts.
Exp Cell Res
; 317(6): 737-44, 2011 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-21237152
ABSTRACT
Fluid shear stress regulates gene expression in osteoblasts, in part by activation of the transcription factor NF-κB. We examined whether this process was under the control of purinoceptor activation. MC3T3-E1 osteoblasts under static conditions expressed the NF-κB inhibitory protein IκBα and exhibited cytosolic localization of NF-κB. Under fluid shear stress, IκBα levels decreased, and concomitant nuclear localization of NF-κB was observed. Cells exposed to fluid shear stress in ATP-depleted medium exhibited no significant reduction in IκBα, and NF-κB remained within the cytosol. Similar results were found using oxidized ATP or Brilliant Blue G, P2X(7) receptor antagonists, indicating that the P2X(7) receptor is responsible for fluid shear-stress-induced IκBα degradation and nuclear accumulation of NF-κB. Pharmacologic blockage of the P2Y6 receptor also prevented shear-induced IκBα degradation. These phenomena involved neither ERK1/2 signaling nor autocrine activation by P2X(7)-generated lysophosphatidic acid. Our results suggest that fluid shear stress regulates NF-κB activity through the P2Y(6) and P2X(7) receptor.
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1
Banco de datos:
MEDLINE
Asunto principal:
Osteoblastos
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Estrés Mecánico
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Transducción de Señal
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FN-kappa B
Límite:
Animals
Idioma:
En
Año:
2011
Tipo del documento:
Article