Corneal epithelialisation on surface-modified hydrogel implants: artificial cornea.
J Mater Sci Mater Med
; 22(3): 663-70, 2011 Mar.
Article
en En
| MEDLINE
| ID: mdl-21287242
ABSTRACT
The objective was to investigate corneal re-epithelialisation of surface-modified polymethacrylate hydrogel implants in order to evaluate them as potential materials for an artificial cornea. Polymethacrylate hydrogels were modified with amines and then coated with different extracellular matrix proteins (collagen I, IV, laminin and fibronectin). The modified hydrogels were surgically implanted into bovine corneas maintained in a 3-D culture system for 5 days. The epithelial growth across the implant surface was evaluated using fluorescent, light and electron microscopy. Full epithelialisation was achieved on 1,4-diaminobutane-modified hydrogels after coating with collagen IV. Hydrogels modified with 1,4-diaminobutane but without further coating only showed partial re-epithelialisation. Hydrogels modified with other amines (1,2-diaminoethane or 1,3-diaminopropane) showed only partial re-epithelialisation; further coating with extracellular matrix proteins improved epithelialisation of these surfaces but did not result in complete re-epithelialisation. Evaluation of the corneas implanted with the 1,4-diaminobutane-modified hydrogels coated with collagen IV showed that the artificial corneas remain clear, integrate well and become covered by a healthy stratified epithelium. In conclusion the 1,4-diaminobutane surface-modified hydrogel coated with collagen IV supported the growth of a stable stratified epithelium. With further refinement this hydrogel has the potential to be used clinically for an artificial cornea.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Prótesis e Implantes
/
Epitelio Corneal
/
Córnea
/
Hidrogel de Polietilenoglicol-Dimetacrilato
Límite:
Humans
Idioma:
En
Año:
2011
Tipo del documento:
Article