Growth factors induce phosphorylation of the Na+/H+ antiporter, glycoprotein of 110 kD.
Science
; 247(4943): 723-6, 1990 Feb 09.
Article
en En
| MEDLINE
| ID: mdl-2154036
ABSTRACT
The Na+/H+ antiporter, which regulates intracellular pH in virtually all cells, is one of the best examples of a mitogen- and oncogene-activated membrane target whose activity rapidly changes on stimulation. The activating mechanism is unknown. A Na+/H+ antiporter complementary DNA fragment was expressed in Escherichia coli as a beta-galactosidase fusion protein, and a specific antibody to the fusion protein was prepared. Use of this antibody revealed that the Na+/H+ antiporter is a 110-kilodalton glycoprotein that is phosphorylated in growing cells. Mitogenic activation of resting hamster fibroblasts and A431 human epidermoid cells with epidermal growth factor, thrombin, phorbol esters, or serum, stimulated phosphorylation of the Na+/H+ antiporter with a time course similar to that of the rise in intracellular pH.
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Banco de datos:
MEDLINE
Asunto principal:
Proteínas Portadoras
/
Sustancias de Crecimiento
Límite:
Animals
/
Humans
Idioma:
En
Año:
1990
Tipo del documento:
Article