Essential roles of snap-29 in C. elegans.
Dev Biol
; 355(1): 77-88, 2011 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-21545795
ABSTRACT
SNARE domain proteins are key molecules mediating intracellular fusion events. SNAP25 family proteins are unique target-SNAREs possessing two SNARE domains. Here we report the genetic, molecular, and cell biological characterization of C. elegans SNAP-29. We found that snap-29 is an essential gene required throughout the life-cycle. Depletion of snap-29 by RNAi in adults results in sterility associated with endomitotic oocytes and pre-meiotic maturation of the oocytes. Many of the embryos that are produced are multinucleated, indicating a defect in embryonic cytokinesis. A profound defect in secretion by oocytes and early embryos in animals lacking SNAP-29 appears to be the underlying defect connecting these phenotypes. Further analysis revealed defects in basolateral and apical secretion by intestinal epithelial cells in animals lacking SNAP-29, indicating a broad requirement for this protein in the secretory pathway. A SNAP-29-GFP fusion protein was enriched on recycling endosomes, and loss of SNAP-29 disrupted recycling endosome morphology. Taken together these results suggest a requirement for SNAP-29 in the fusion of post-Golgi vesicles with the recycling endosome for cargo to reach the cell surface.
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1
Banco de datos:
MEDLINE
Asunto principal:
Endosomas
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Caenorhabditis elegans
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Proteínas de Caenorhabditis elegans
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Proteínas SNARE
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Proteínas Q-SNARE
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Aparato de Golgi
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Infertilidad
Límite:
Animals
Idioma:
En
Año:
2011
Tipo del documento:
Article