Quantification of human serum procollagen C-proteinase enhancer (hsPCPE) glycopattern.
Clin Chim Acta
; 412(19-20): 1762-6, 2011 Sep 18.
Article
en En
| MEDLINE
| ID: mdl-21569766
ABSTRACT
BACKGROUND:
Procollagen C-proteinase enhancer 1 (PCPE1), a glycoprotein secreted from differentiating osteoblast, enhances the rate-limiting step of collagen type I fibrillar formation. It is expressed and secreted by cells that produce collagen type I and has the potential to be a marker for bone pathologies.METHODS:
We developed an assay to quantify PCPE glycopattern based on isoelectric focusing (IEF) and detection with a bio-imaging camera (coefficient of variation within and between assays, 15% and 20%, respectively).RESULTS:
PCPE was quantified in 39 serum samples from healthy subjects (17 females and 22 males). The concentration in the serum was 305(274) ng/ml, median(IQR). The level of the PCPE isoforms and their relative distribution were altered in patients with bone disorders.CONCLUSIONS:
The data generated by our system, support our hypothesis that combined data on PCPE concentration and isoforms may be useful for the diagnosis and follow-up of bone diseases. Further research, on larger cohorts of both normal subjects and patients, must be done.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Carbohidratos
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Glicoproteínas
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Proteínas de la Matriz Extracelular
Límite:
Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Año:
2011
Tipo del documento:
Article