A hypomorphic IgH-chain allele affects development of B-cell subsets and favours receptor editing.
EMBO J
; 30(13): 2705-18, 2011 May 27.
Article
en En
| MEDLINE
| ID: mdl-21623346
ABSTRACT
The quality and quantity of BCR signals impact on cell fate decisions of B lymphocytes. Here, we describe novel gene-targeted mice, which in the context of normal VDJ recombination show hypomorphic expression of immunoglobulin µ heavy chain (µHC) mRNA levels and hence lower pre-BCR and BCR levels. Hypomorphic expression of µHC leads to augmented selection processes at all stages of B-cell development, noticeably at the expansion of pre-B cells, the positive selection of immature B lymphocytes in the bone marrow and the selection of the follicular (FO), marginal zone (MZ) and B1 B-lymphocyte compartment in peripheral lymphoid organs. Immature as well as mature FO and MZ B lymphocytes in the peripheral lymphoid organs express lower levels of the receptor for B-cell activating factor (BAFF). In addition, hypomorphic expression of the BCR favours receptor editing. Together, our results highlight the critical importance of pre-BCR and BCR receptor levels for the normal development of B-lymphocyte subpopulations in the context of intact VDJ recombination and a diverse antibody repertoire.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Receptores de Antígenos de Linfocitos B
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Diferenciación Celular
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Subgrupos de Linfocitos B
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Genes de las Cadenas Pesadas de las Inmunoglobulinas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Año:
2011
Tipo del documento:
Article