Production, purification and characterization of a thermostable ß-1,3-glucanase produced by Moniliophthora perniciosa.
An Acad Bras Cienc
; 83(2): 599-609, 2011 Jun.
Article
en En
| MEDLINE
| ID: mdl-21625799
ABSTRACT
The enzyme glucanase from Moniliophthora perniciosa was produced in liquid medium and purified from the culture supernatant. A multivariate statistical approach (Response Surface Methodology - RSM) was employed to evaluate the effect of variables, including inducer (yeast extract) and fermentation time, on secreted glucanase activities M. perniciosa detected in the culture medium. The crude enzyme present in the supernatant was purified in two steps:
precipitation with ammonium sulfate (70%) and gel filtration chromatography on Sephacryl S-200. The best inducer and fermentation time for glucanase activities were 5.9 g L(-1) and 13 days, respectively. The results revealed three different isoforms (GLUI, GLUII and GLUIII) with purification factors of 4.33, 1.86 and 3.03, respectively. The partially purified enzymatic extract showed an optimum pH of 5.0 and an optimum temperature of 40°C. The enzymatic activity increased in the presence of KCl at all concentrations studied. The glucanase activity was highest in the presence of 0.2 M NaCl. The enzyme showed high thermal stability, losing only 10.20% of its specific activity after 40 minutes of incubation at 90°C. A purified enzyme with relatively good thermostability that is stable at low pH might be used in future industrial applications.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Agaricales
/
Glucano Endo-1,3-beta-D-Glucosidasa
Idioma:
En
Año:
2011
Tipo del documento:
Article