Serum antibodies to the HPV16 proteome as biomarkers for head and neck cancer.
Br J Cancer
; 104(12): 1896-905, 2011 Jun 07.
Article
en En
| MEDLINE
| ID: mdl-21654689
ABSTRACT
BACKGROUND:
Human papillomavirus (HPV) type 16 is associated with oropharyngeal carcinomas (OPC). Antibodies (Abs) to HPV16 E6 and E7 oncoproteins have been detected in patient sera; however, Abs to other early HPV-derived proteins have not been well explored.METHODS:
Antibodies to the HPV16 proteome were quantified using a novel multiplexed bead assay, using C-terminal GST-fusion proteins captured onto Luminex beads. Sera were obtained from untreated patients with OPC (N=40), partners of patients with HPV16+ OPC (N=11), and healthy controls (N=50).RESULTS:
Oropharyngeal carcinomas patients with known virus-like capsid particle+ Abs had elevated serum Abs to HPV16 E1, E2, E4, E6, and E7, and L1 antibody levels, but not E5. The ratios of specific median fluorescence intensity to p21-GST compared with controls were E1 50.7 vs 2.1; E4 14.6 vs 1.3; E6 11.3 vs 2.4; E7 43.1 vs 2.6; and L1 10.3 vs 2.6 (each P≤0.01). In a validation cohort, HPV16 E1, E2, and E7 antibody levels were significantly elevated compared with healthy control samples (P≤0.02) and partners of OPC patients (P≤0.01).CONCLUSION:
Patients with HPV16+ OPC have detectable Abs to E1, E2, and E7 proteins, which are potential biomarkers for HPV-associated OPC.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Represoras
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Neoplasias Orofaríngeas
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Biomarcadores de Tumor
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Proteínas Oncogénicas Virales
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Proteoma
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Papillomavirus Humano 16
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Proteínas E7 de Papillomavirus
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Anticuerpos Antivirales
Tipo de estudio:
Diagnostic_studies
Límite:
Aged
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Aged80
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Año:
2011
Tipo del documento:
Article