[Simultaneous determination of six flavonoids in Apocynum venetum by HPLC].
Zhongguo Zhong Yao Za Zhi
; 36(5): 589-93, 2011 Mar.
Article
en Zh
| MEDLINE
| ID: mdl-21657078
ABSTRACT
OBJECTIVE:
To develop a high-performance liquid chromatography (HPLC) method for the simultaneous determination of rutin, hyperoside, isoquercetin, astragulin, quercetin, and kaempferol in Apocynum venetum and its extracts.METHOD:
The separation was carried out on a Shim pack ODS (4.6 mm x 250 mm, 5 microm) colum eluted with in mobile phases of water containing 0.2% phosphoric acid and acetonitrile containing 0.2% phosphoric acid in acetonitrile gradient mode. The column temperature was 40 degrees C, and the flow rate was 1.0 mL x min(-1). The detection wavelength was set at 360 nm.RESULT:
The good seperation of six flavonoids was achieved within 40 min, with the relative standard deviations (RSD) of intra- and inter-day precision < or = 2.0%. Calibration curves of rutin, hyperoside, isoquercetin, astragulin, quercetin, and kaempferol showed good linear relationship (R2 > 0.999 7, n = 6). The average recoveries of the six flavonoids were within 97.30% - 105.8% (RSD 2.6%). Three batches of A. venetum and 2 batches of its extracts were determined.CONCLUSION:
The developed method is simple, accurate, and repeatable, and can be readily used as a powerful tool for the quality control of A. venetum and its extracts.
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Banco de datos:
MEDLINE
Asunto principal:
Flavonoides
/
Cromatografía Líquida de Alta Presión
/
Apocynum
Idioma:
Zh
Año:
2011
Tipo del documento:
Article