Receptor-mediated action of hepoxilin A3 releases diacylglycerol and arachidonic acid from human neutrophils.
Biochem Biophys Res Commun
; 171(3): 944-8, 1990 Sep 28.
Article
en En
| MEDLINE
| ID: mdl-2171511
ABSTRACT
We have previously shown that hepoxilin A3 increases the intracellular concentration of Ca+2 in human neutrophils. Herein we address the initial events of hepoxilin action on the neutrophil which precede the rise in intracellular calcium. We show that hepoxilin A3 at 10-1000 nM concentrations releases from [1-14C]-arachidonic acid labeled neutrophils diacylglycerol and unesterified arachidonic acid in a time and concentration dependent fashion. The release of arachidonic acid and diacyglycerol are receptor-mediated events which are blocked by pertussis toxin. This data shows that hepoxilin A3 stimulates phospholipases C and A2 in the cell which may be involved in the rise in cytosolic calcium. Thus, hepoxilins may represent a hitherto unrecognised class of cellular mediators.
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Banco de datos:
MEDLINE
Asunto principal:
Ácidos Araquidónicos
/
Diglicéridos
/
Ácido 8,11,14-Eicosatrienoico
/
Neutrófilos
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Año:
1990
Tipo del documento:
Article