Development of a real-time RT-PCR method for rapid detection of H9 avian influenza virus in the air.
Arch Virol
; 156(10): 1795-801, 2011 Oct.
Article
en En
| MEDLINE
| ID: mdl-21735211
ABSTRACT
Avian influenza virus (AIV) has caused serious epidemics all over the world. Notably, the low-pathogenic AIV H9N2 has been spreading widely, leading to enormous economic losses to the poultry industry. To rapidly monitor airborne H9 AIVs in chicken houses, a real-time RT-PCR method was established and used to detect virus in air samples, and it was also compared with the traditional RT-PCR. The results showed that the real-time RT-PCR possessed high specificity and sensitivity for H9 AIVs, and the sensitivity reached 100 copies/reaction, much higher than the traditional RT-PCR; airborne H9 AIVs were found in the six chicken houses by real-time RT-PCR, and their mean concentrations ranged from 1.25×10(4) to 6.92×10(4) copies/m(3) air. Overall, the real-time PCR is a valuable tool for detecting airborne H9 AIVs.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Enfermedades de las Aves de Corral
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Microbiología del Aire
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Subtipo H9N2 del Virus de la Influenza A
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Gripe Aviar
Tipo de estudio:
Diagnostic_studies
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Evaluation_studies
Límite:
Animals
Idioma:
En
Año:
2011
Tipo del documento:
Article