Biomarkers of organophosphorus (OP) exposures in humans.
Neurotoxicology
; 32(5): 656-60, 2011 Oct.
Article
en En
| MEDLINE
| ID: mdl-21767566
ABSTRACT
There are ongoing events where aircraft engine lubricant containing tricresyl phosphates (TCPs) contaminates aircraft cabins. Some individuals have experienced tremors or other neurological symptoms that may last for many months following exposures. Mass spectrometric (MS) protocols are being developed to determine the percentage of "biomarker proteins" that are modified by such exposures, specifically on active site serines. Both plasma butyrylcholinesterase (BChE) and red cell acylpeptide hydrolase (APH) are readily inhibited by 2-(ortho-cresyl)-4H-1,3,2-benzodioxaphosphoran-2-one (CBDP) or phenyl saligenin cyclic phosphate (PSP) and have the potential to provide information about the level of exposure of an individual. We have developed immunomagnetic bead-based single-step purification protocols for both BChE and APH and have characterized the active site serine adducts of BChE by MS.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Compuestos Organofosforados
/
Exposición a Riesgos Ambientales
Límite:
Humans
Idioma:
En
Año:
2011
Tipo del documento:
Article