Intranasal immunization with LACK-DNA promotes protective immunity in hamsters challenged with Leishmania chagasi.
Parasitology
; 138(14): 1892-7, 2011 Dec.
Article
en En
| MEDLINE
| ID: mdl-21867591
ABSTRACT
LACK (Leishmania analogue of the receptor kinase C) is a conserved protein in protozoans of the genus Leishmania which is associated with the immunopathogenesis and susceptibility of BALB/c mice to L. major infection. Previously, we demonstrated that intranasal immunization with a plasmid carrying the LACK gene of Leishmania infantum (LACK-DNA) promotes protective immunity in BALB/c mice against Leishmania amazonensis and Leishmania chagasi. In the present study, we investigated the protective immunity achieved in hamsters intranasally vaccinated with 2 doses of LACK-DNA (30 µg). Compared with controls (PBS and pCI-neo plasmid), animals vaccinated with LACK-DNA showed significant reduction in parasite loads in the spleen and liver, increased lymphoproliferative response and increased nitric oxide (NO) production by parasite antigen-stimulated splenocytes. Furthermore, hamsters vaccinated with LACK-DNA presented high IgG and IgG2a serum levels when compared to control animals. Our results showed that intranasal vaccination with LACK-DNA promotes protective immune responses in hamsters and demonstrated the broad spectrum of intranasal LACK-DNA efficacy in different host species, confirming previous results in murine cutaneous and visceral leishmaniasis.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
ADN Protozoario
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Vacunación
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Leishmania infantum
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Vacunas contra la Leishmaniasis
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Leishmania
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Leishmaniasis Visceral
Límite:
Animals
Idioma:
En
Año:
2011
Tipo del documento:
Article