On-column solvent exchange for purified preparative fractions.
J Lab Autom
; 16(5): 335-46, 2011 Oct.
Article
en En
| MEDLINE
| ID: mdl-21906559
ABSTRACT
On-column solvent exchange, using many of the principles of solid-phase extraction, has been implemented to significantly reduce evaporation cycle time following reverse-phase preparative HPLC. Additional benefits, such as a reduced potential for salt formation, thermal decomposition, and residual solvent, are also described. Fractions obtained from preparative separations, typically in a large volume of acetonitrilewater, are injected into the preparative HPLC and then eluted in acetonitrile, creating a new fraction in a volatile organic solvent. Minimal modification to the instrument was required, and unattended operation is possible. Acetonitrile evaporation is achieved within 3 h, compared with 17 h for aqueous-based fractions; lower temperatures can be used during the evaporation step; mobile-phase additives, likely to form salts with the target compound if concentrated in the fraction, are removed before evaporation; sample recovery and purity are unaffected.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Acetonitrilos
/
Solventes
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Cromatografía Líquida de Alta Presión
Idioma:
En
Año:
2011
Tipo del documento:
Article