Differentiating ovine BSE from CH1641 scrapie by serial protein misfolding cyclic amplification.
Mol Biotechnol
; 51(3): 233-9, 2012 Jul.
Article
en En
| MEDLINE
| ID: mdl-21987099
ABSTRACT
Whilst ovine BSE displays distinct pathological characteristics to ovine CH1641-like scrapie upon passage in rodents, they have very similar molecular phenotypes. As such, the in vitro differentiation of these strains in routine surveillance programmes presents a significant diagnostic challenge. In this study, using serial protein-misfolding cyclic amplification (sPMCA), ovine BSE was readily amplified in vitro in brain substrates from sheep with V136R154Q171/V136R154Q171 or AHQ/AHQ PRNP genotypes. In contrast, the CH1641 strain was refractory to such amplification. This method allowed for complete and unequivocal differentiation of experimental BSE from CH1641 prion strains within an ovine host.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Scrapie
/
Encefalopatía Espongiforme Bovina
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Tipificación Molecular
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Año:
2012
Tipo del documento:
Article