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Toll-like receptor 7 controls the anti-retroviral germinal center response.
Browne, Edward P.
  • Browne EP; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America. ebrowne@mit.edu
PLoS Pathog ; 7(10): e1002293, 2011 Oct.
Article en En | MEDLINE | ID: mdl-21998589
ABSTRACT
The development of vaccines that can enhance immunity to viral pathogens is an important goal. However, the innate molecular pathways that regulate the strength and quality of the immune response remain largely uncharacterized. To define the role of Toll-like receptor (TLR) signaling in control of a model retroviral pathogen, Friend virus (FV), I generated mice in which the TLR signaling adapter Myd88 was selectively deleted in dendritic cell (DC) or in B cell lineages. Deletion of Myd88 in DCs had little effect on immune control of FV, while B cell specific deletion of Myd88 caused a dramatic increase in viral infectious centers and a significantly reduced antibody response, indicating that B cell-intrinsic TLR signaling plays a crucial role, while TLR signaling in DCs is less important. I then identified the single-stranded RNA sensing protein TLR7 as being required for antibody-mediated control of FV by analyzing mice deficient in TLR7. Remarkably, B cells in infected TLR7-deficient mice upregulated CD69 and CD86 early in infection, but failed to develop into germinal center B cells. CD4 T cell responses were also attenuated in the absence of TLR7, but CD8 responses were TLR7 independent, suggesting the existence of additional pathways for detection of retroviral particles. Together these results demonstrate that the vertebrate immune system detects retroviruses in vivo via TLR7 and that this pathway regulates a key checkpoint controlling development of germinal center B cells.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Glicoproteínas de Membrana / Infecciones por Retroviridae / Centro Germinal / Receptor Toll-Like 7 Tipo de estudio: Prognostic_studies Idioma: En Año: 2011 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Glicoproteínas de Membrana / Infecciones por Retroviridae / Centro Germinal / Receptor Toll-Like 7 Tipo de estudio: Prognostic_studies Idioma: En Año: 2011 Tipo del documento: Article