Computation-guided backbone grafting of a discontinuous motif onto a protein scaffold.
Science
; 334(6054): 373-6, 2011 Oct 21.
Article
en En
| MEDLINE
| ID: mdl-22021856
ABSTRACT
The manipulation of protein backbone structure to control interaction and function is a challenge for protein engineering. We integrated computational design with experimental selection for grafting the backbone and side chains of a two-segment HIV gp120 epitope, targeted by the cross-neutralizing antibody b12, onto an unrelated scaffold protein. The final scaffolds bound b12 with high specificity and with affinity similar to that of gp120, and crystallographic analysis of a scaffold bound to b12 revealed high structural mimicry of the gp120-b12 complex structure. The method can be generalized to design other functional proteins through backbone grafting.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Anticuerpos Anti-VIH
/
Ingeniería de Proteínas
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Proteína gp120 de Envoltorio del VIH
/
Anticuerpos Neutralizantes
Idioma:
En
Año:
2011
Tipo del documento:
Article