Application of long synthetic oligonucleotides for gene analysis: effect of probe length and stringency conditions on hybridization specificity.

Kajimura, Y; Krull, J; Miyakoshi, S; Itakura, K; Toyoda, H

Kajimura, Y; Krull, J; Miyakoshi, S; Itakura, K; Toyoda, H.

Kajimura Y; Department of Molecular Genetics, Beckman Research Institute of the City of Hope, Duarte, CA.

Genet Anal Tech Appl ; 7(4): 71-9, 1990 Jun.

Article en En | MEDLINE | ID: mdl-2206600

ABSTRACT

ABSTRACT

Two different lengths of long unique synthetic oligonucleotide probes (37- and 48-mers) specific for human major histocompatibility complex (MHC) class II beta genes were synthesized. These oligonucleotides were utilized to examine factors influencing hybridization specificity. Both probe length and stringency of washing conditions were found to be crucial factors for sequence-specific hybridization.

Asunto(s)

Antígenos HLA-DQ/genética; Antígenos HLA-DR/genética; Hibridación de Ácido Nucleico; Sondas de Oligonucleótidos; Alelos; Secuencia de Aminoácidos; Secuencia de Bases; Línea Celular; ADN/análisis; Humanos; Datos de Secuencia Molecular; Homología de Secuencia de Ácido Nucleico; Temperatura

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Banco de datos: MEDLINE Asunto principal: Sondas de Oligonucleótidos / Antígenos HLA-DQ / Antígenos HLA-DR / Hibridación de Ácido Nucleico Límite: Humans Idioma: En Año: 1990 Tipo del documento: Article

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