Association between single-nucleotide polymorphisms of DNMT3L and infertility with azoospermia in Chinese men.
Reprod Biomed Online
; 24(1): 66-71, 2012 Jan.
Article
en En
| MEDLINE
| ID: mdl-22116073
ABSTRACT
The gene for DNA methyltransferase 3-like protein (DNMT3L) is essential for normal spermatogenesis and may be involved with spermatogenetic impairment and male infertility. To explore the possible association between the DNMT3L gene and male infertility, this study investigated allele, genotype and haplotype frequencies of three single nucleotide polymorphism (SNP) loci, rs2070565, rs2276248 and rs7354779, of DNMT3L in 233 infertile patients with azoospermia and 249 fertile controls from a population of Chinese men using polymerase chain reaction/restriction fragment length polymorphism. Results showed that the frequencies of allele A (20.6% versus 14.9%; P = 0.022) and the allele A carrier (GA + AA; 37.8% versus 28.1%; P = 0.027) in azoospermic patients were significantly higher than those in controls at the rs2070565 locus. The haplotype AAA frequency was significantly higher (18.1% versus 12.4%; P = 0.02) while the haplotype GAA frequency was significantly lower (53.2% versus 62.1%; P = 0.007) in infertile patients compared with fertile controls. These results indicated that SNP rs2070565, as well as haplotypes AAA and GAA, may be associated with male infertility and suggest that DNMT3L may contribute to azoospermia susceptibility in humans.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Metilación de ADN
/
Polimorfismo de Nucleótido Simple
/
ADN (Citosina-5-)-Metiltransferasas
/
Azoospermia
Tipo de estudio:
Risk_factors_studies
Límite:
Adult
/
Humans
/
Male
País como asunto:
Asia
Idioma:
En
Año:
2012
Tipo del documento:
Article