Suppression of nasopharyngeal carcinoma cell by targeting ß-catenin signaling pathway.
Cancer Epidemiol
; 36(2): e116-21, 2012 Apr.
Article
en En
| MEDLINE
| ID: mdl-22142772
ABSTRACT
AIM:
To investigate the role of ß-catenin in pathogenesis of nasopharyngeal carcinoma (NPC).METHODS:
Cellular proliferation, apoptosis, matrix penetration assay, and western blotting were employed to determine cell biological changes in NPC cell lines transfected with ß-catenin siRNA. Immunohistochemistry staining was used to detect ß-catenin and Ki-67 expression in NPC tissue.RESULTS:
ß-Catenin was upregulated in NPC cell lines and tissues compared with chronic nasopharyngitis tissue. ß-Catenin knockdown dramatically inhibited cellular growth, migration and invasion, but induced apoptosis of NPC cells. Further study showed that downstream genes of ß-catenin signaling pathway including cyclin D1, c-Myc, MMP2 and MMP9 expression were suppressed in NPC cell lines transfected with ß-catenin siRNA.CONCLUSION:
Targeting ß-catenin signaling pathway may be a noval strategy for NPC therapy.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Transducción de Señal
/
Neoplasias Nasofaríngeas
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Beta Catenina
Límite:
Adolescent
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Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Año:
2012
Tipo del documento:
Article