E-cadherin and caveolin-1 alterations in the heart of rats having undergone chlorpromazine-induced toxicity.
Mol Med Rep
; 5(3): 705-9, 2012 03.
Article
en En
| MEDLINE
| ID: mdl-22200730
ABSTRACT
Heart damage induced by chlorpromazine (CPZ) toxicity is associated with changes in the expression of various enzymes and proteins. This study aimed to investigate CPZinduced alterations in cardiac E-cadherin and caveolin-1 (cav-1) after CPZ administration. Male Wistar rats were randomly divided into two groups a control group and a CPZ group. The CPZ group was administered CPZ intraperitoneally at a single dose of 10 mg/kg for 21 days; the controls were given the same amount of saline via the same route. On Day 22, the rats were anesthetized, and a thoracotomy was performed in all animals. Immunohistochemical analysis was performed to evaluate protein expression of E-cadherin and cav-1. Sections were analyzed by digital image analysis. Results of the present study revealed that cardiac protein expression of E-cadherin and cav-1 was altered after CPZ-induced toxicity in the rat. The expression of E-cadherin was significantly reduced, while expression of cav-1 was significantly increased after CPZ treatment, as supported by integrated optical density analysis, compared with the control (P<0.05). The current findings indicate that such changes in the expression of E-cadherin and cav-1 may be reflected in abnormal cardiac function, and these proteins may be useful in revealing the mechanisms underlying CPZ-induced toxicity and may also provide additional insight for further research.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Cadherinas
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Clorpromazina
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Regulación de la Expresión Génica
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Caveolina 1
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Corazón
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Antieméticos
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Miocardio
Límite:
Animals
Idioma:
En
Año:
2012
Tipo del documento:
Article