Rapid alkaline transfer of low molecular weight DNA from NuSieve GTG agarose gels.
Biotechniques
; 9(3): 260-2, 1990 Sep.
Article
en En
| MEDLINE
| ID: mdl-2223064
ABSTRACT
The rapid alkaline transfer of high molecular weight DNA from agarose gels to nylon membranes has greatly decreased the time required for setup of Southern transfers. This technique has been used to resolve genomic DNA greater than 1000 base pairs by conventional electrophoresis on 1% agarose gels followed by alkaline transfer to nylon membrane. Now we report that this rapid alkaline method can be used for the transfer of low molecular weight DNA fragments (10 to 1000 base pairs) from NuSieve GTG agarose gels to nylon membrane.
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Banco de datos:
MEDLINE
Asunto principal:
Sefarosa
/
ADN
Límite:
Humans
Idioma:
En
Año:
1990
Tipo del documento:
Article