Necrotising enterocolitis is characterised by disrupted immune regulation and diminished mucosal regulatory (FOXP3)/effector (CD4, CD8) T cell ratios.
Gut
; 62(1): 73-82, 2013 Jan.
Article
en En
| MEDLINE
| ID: mdl-22267598
ABSTRACT
BACKGROUND:
Necrotising enterocolitis (NEC) is the most common gastrointestinal emergency in premature infants. Immaturity of gastrointestinal immune regulation may predispose preterm infants to NEC as FOXP3 T regulatory cells (Treg) are critical for intestinal immune homoeostasis.OBJECTIVE:
To investigate the hypothesis that abnormal developmental regulation of lamina propria Treg would define premature infants with NEC.DESIGN:
Lamina propria mononuclear cell populations from surgically resected ileum from 18 patients with NEC and 30 gestational age-matched non-NEC surgical controls were prospectively isolated. Polychromatic flow cytometry was performed to phenotype and analyse lamina propria T cell populations. The cytokine gene expression profile in NEC tissue was compared with that of non-NEC controls.RESULTS:
The total number of Treg, CD4, or CD8 T cells in each ileum section was independent of gestational age, age or postmenstrual age and similar between patients with NEC and controls. In contrast, the ratio of Treg to CD4 T cells or Treg to CD8 T cells was significantly lower in NEC ileum than in infants without NEC (medians 2.9% vs 6.6%, p=0.001 and medians 6.6% vs 25.9%, p<0.001, respectively). For any given number of CD4 or CD8 T cells, Treg were, on average, 60% lower in NEC ileum than in controls. NEC tissue cytokine gene expression profiles were characteristic of inhibited Treg development or function. Treg/CD4 and Treg/CD8 ratios recovered between initial resection for NEC and reanastomosis.CONCLUSION:
The proportion of lamina propria Treg is significantly reduced in the ileum of premature infants with NEC and may contribute to the excessive inflammatory state of this disease.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Linfocitos T Reguladores
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Linfocitos T CD8-positivos
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Enterocolitis Necrotizante
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Factores de Transcripción Forkhead
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Enfermedades del Prematuro
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Mucosa Intestinal
Tipo de estudio:
Observational_studies
Límite:
Female
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Humans
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Male
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Newborn
Idioma:
En
Año:
2013
Tipo del documento:
Article