[Identification of genetically modified vegetable sources in food and feed using hydrogel oligonucleotide microchip].
Mol Biol (Mosk)
; 45(6): 973-83, 2011.
Article
en Ru
| MEDLINE
| ID: mdl-22295567
ABSTRACT
A method of multiplex polymerase chain reaction (PCR) followed by the hybridization on a hydrogel oligonucleotide biochip was developed for simultaneous identification of ten different transgenic elements of plant DNA in feed and food products. The biochip contained 22 immobilized probes intended for (i) detection of plant DNA; (ii) plant species determination (soybean, maize, potato, rice); (iii) identification of transgenic elements, including 35S CaMV, 35S FMV, rice actine gene promoters, nos, 35S CaMV, ocs, pea rbcS1 gene terminators, and bar, gus, nptII marker genes. The limit of detection was 0.5% of genetically modified (GM) soybean and maize in analyzed samples. Identification of transgenic DNA in food and feed products using either the developed approach or real-time PCR led to virtually identical results. The assay can be used for selection of GM samples by screening food and feed products for subsequent quantitative determination of the GM component based on the identified transgene.
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Banco de datos:
MEDLINE
Asunto principal:
Verduras
/
Reacción en Cadena de la Polimerasa
/
Plantas Modificadas Genéticamente
/
ADN de Plantas
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Análisis de los Alimentos
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
Ru
Año:
2011
Tipo del documento:
Article