Synthesis and validation of cyanine-based dyes for DIGE.
Methods Mol Biol
; 854: 67-85, 2012.
Article
en En
| MEDLINE
| ID: mdl-22311754
ABSTRACT
The application of difference gel electrophoresis (DIGE), in particular its most common "minimal labeling" variety, utilizes N-hydroxysuccinimide esters of Cy2, Cy3, and Cy5 dyes, which are commercially available. We describe methods for the efficient synthesis of all three dyes from relatively inexpensive and commercially available precursors in only a few steps and with relatively high yields. In model DIGE experiments, the newly synthesized dyes proved to be indistinguishable from commercially available ones and have been shown to be stable for years while stored under argon as dry solids or after being dissolved in N,N-dimethylformamide.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Carbocianinas
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Colorantes
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Electroforesis Bidimensional Diferencial en Gel
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Técnicas de Química Sintética
Tipo de estudio:
Prognostic_studies
Idioma:
En
Año:
2012
Tipo del documento:
Article