Polysaccharide films built by simultaneous or alternate spray: a rapid way to engineer biomaterial surfaces.
Langmuir
; 28(22): 8470-8, 2012 Jun 05.
Article
en En
| MEDLINE
| ID: mdl-22554108
ABSTRACT
We investigated polysaccharide films obtained by simultaneous and alternate spraying of a chitosan (CHI) solution as polycation and hyaluronic acid (HA), alginate (ALG), and chondroitin sulfate (CS) solutions as polyanions. For simultaneous spraying, the film thickness increases linearly with the cumulative spraying time and passes through a maximum for polyanion/CHI molar charge ratios lying between 0.6 and 1.2. The size of polyanion/CHI complexes formed in solution was compared with the simultaneously sprayed film growth rate as a function of the polyanion/CHI molar charge ratio. A good correlation was found. This suggests the importance of polyanion/polycation complexation in the simultaneous spraying process. Depending on the system, the film topography is either liquid-like or granular. Film biocompatibility was evaluated using human gingival fibroblasts. A small or no difference is observed in cell viability and adhesion between the two deposition processes. The CHI/HA system appears to be the best for cell adhesion inducing the clustering of CD44, a cell surface HA receptor, at the membrane of cells. Simultaneous or alternate spraying of CHI/HA appears thus to be a convenient and fast procedure for biomaterial surface modifications.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Poliaminas
/
Polímeros
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Materiales Biocompatibles
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Sulfatos de Condroitina
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Quitosano
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Alginatos
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Ácido Hialurónico
Límite:
Humans
Idioma:
En
Año:
2012
Tipo del documento:
Article