Joint Bayesian inference of condition-specific miRNA and transcription factor activities from combined gene and microRNA expression data.
Bioinformatics
; 28(13): 1714-20, 2012 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-22563068
ABSTRACT
MOTIVATION There have been many successful experimental and bioinformatics efforts to elucidate transcription factor (TF)-target networks in several organisms. For many organisms, these annotations are complemented by miRNA-target networks of good quality. Attempts that use these networks in combination with gene expression data to draw conclusions on TF or miRNA activity are, however, still relatively sparse. RESULTS:
In this study, we propose Bayesian inference of regulation of transcriptional activity (BIRTA) as a novel approach to infer both, TF and miRNA activities, from combined miRNA and mRNA expression data in a condition specific way. That means our model explains mRNA and miRNA expression for a specific experimental condition by the activities of certain miRNAs and TFs, hence allowing for differentiating between switches from active to inactive (negative switch) and inactive to active (positive switch) forms. Extensive simulations of our model reveal its good prediction performance in comparison to other approaches. Furthermore, the utility of BIRTA is demonstrated at the example of Escherichia coli data comparing aerobic and anaerobic growth conditions, and by human expression data from pancreas and ovarian cancer. AVAILABILITY AND IMPLEMENTATION The method is implemented in the R package birta, which is freely available for Bio-conductor (>=2.10) on http//www.bioconductor.org/packages/release/bioc/html/birta.html.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
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Perfilación de la Expresión Génica
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MicroARNs
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Redes Reguladoras de Genes
Tipo de estudio:
Prognostic_studies
Límite:
Female
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Humans
Idioma:
En
Año:
2012
Tipo del documento:
Article