A naturally processed HLA-DR-bound peptide from the IL-9 receptor alpha of HTLV-1-transformed T cells serves as a T helper epitope.
Cancer Immunol Immunother
; 61(12): 2215-25, 2012 Dec.
Article
en En
| MEDLINE
| ID: mdl-22638550
ABSTRACT
Human T cell leukemia virus type 1 (HTLV-1) induced adult T cell leukemia/lymphoma (ATLL) is usually a fatal lymphoproliferative malignant disease. Thus, the enhancement of T cell immunity to ATLL through the development of therapeutic vaccines using characterized T cell peptide epitopes could be of value. We isolated and characterized HLA-DR-bound peptides from HTLV-1-transformed T cells by fractionating on reverse-phase high performance liquid chromatography and Edman NH(2)-terminal sequencing and were able to identify five independent peptide sequences. One of the identified peptide sequences corresponded to a fragment of the human interleukin-9 receptor alpha (IL-9Rα), which is commonly expressed by HTLV-1-infected T cell lymphoma cells. Using a synthetic peptide corresponding to the identified IL-9Rα sequence, we generated antigen-specific CD4 helper T lymphocytes in vitro, which were restricted by HLA-DR15 or HLA-DR53 molecules and could recognize and kill HTLV-1+, IL-9Rα+ T cell lymphoma cells. These results indicate that IL-9Rα functions as T cell leukemia/lymphoma-associated antigen for CD4 T cells and that synthetic peptides such as the one described here could be used for T cell-based immunotherapy against IL-9Rα positive ATLL.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Virus Linfotrópico T Tipo 1 Humano
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Leucemia-Linfoma de Células T del Adulto
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Antígenos HLA-DR
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Transformación Celular Viral
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Epítopos de Linfocito T
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Receptores de Interleucina-9
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Cadenas HLA-DRB4
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Subtipos Serológicos HLA-DR
Límite:
Animals
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Humans
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Male
Idioma:
En
Año:
2012
Tipo del documento:
Article