CD4(+) CD25(high) Foxp3(+) cells increased in the peritoneal fluid of patients with endometriosis.
Am J Reprod Immunol
; 68(4): 301-8, 2012 Oct.
Article
en En
| MEDLINE
| ID: mdl-22817851
ABSTRACT
PROBLEM:
To evaluate CD4(+) CD25(high) Foxp3(+) cells and IL-6, IL-10, IL-17, and TGF-ß in the peritoneal fluid of women with endometriosis. METHOD OF STUDY A total of ninety-eight patients were studied endometriosis (n = 70) and control (n = 28). First, peritoneal fluid lymphocytes were isolated, and CD4(+) CD25(high) cells were identified using flow cytometry. Then, RT-PCR was performed to verify Foxp3 expression in these cells. Also, IL-6, IL-10, IL-17, and TGF-ß concentration was determined.RESULTS:
Of all the lymphocytes in the peritoneal fluid of women with endometriosis, 36.5% (median) were CD4(+) CD25(high) compared to only 1.15% (median) in the control group (P < 0.001). Foxp3 expression was similarly elevated in patients with the disease compared to those without (median, 50 versus 5; P < 0.001). IL-6 and TGF-ß were higher in endometriosis group (IL-6 327.5 pg/mL versus 195.5 pg/mL; TGF-ß 340 pg/mL versus 171.5 pg/mL; both P < 0.001). IL-10 and IL-17 showed no significant differences between the two groups.CONCLUSION:
The peritoneal fluid of patients with endometriosis had a higher percentage of CD4(+) CD25(high) Foxp3(+) cells and also higher levels of IL-6 and TGF-ß compared to women without the disease. These findings suggest that CD4(+) CD25(high) Foxp3(+) cells may play a role in the pathogenesis of endometriosis.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Líquido Ascítico
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Factor de Crecimiento Transformador beta
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Interleucina-6
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Linfocitos T Reguladores
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Endometriosis
Tipo de estudio:
Prognostic_studies
Límite:
Adult
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Female
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Humans
Idioma:
En
Año:
2012
Tipo del documento:
Article