The impact of distension pressure on acute endothelial cell loss and neointimal proliferation in saphenous vein grafts.
Eur J Cardiothorac Surg
; 42(4): e74-9, 2012 Oct.
Article
en En
| MEDLINE
| ID: mdl-22906599
ABSTRACT
OBJECTIVES:
We aimed to determine the extent of acute endothelial cell loss and neointimal proliferation in the long-term in saphenous vein grafts (SVGs) exposed to defined distension pressures.METHODS:
During routine competence testing of SVGs for coronary artery bypass grafting (CABG), blinded peak pressure measurements were performed in 10 patients. In an experimental set-up, distension pressure-related endothelial damage was studied in the SVGs of 20 patients. In a subgroup (n = 10), each patient's SVG was divided into segments and subjected to four constant pressures (50, 100, 150 and 300 mmHg) for 30 min each. In another subgroup (n = 10), SVGs were exposed to a short phase of high pressure (low pressure followed by 300 mmHg for 5 min). Acute endothelial cell loss was quantified by CD31-immunostaining. After 2 weeks of organ culture, the neointimal proliferation was evaluated using histomorphometry. Pressure-related damage was compared with damage at baseline (0 mmHg).RESULTS:
During routine competence testing for CABG, we revealed a median peak pressure of 355 mmHg (range 240-639 mmHg). In the experimental set-up, significant acute endothelial cell loss occurred at all tested distension pressures at 50 mmHg, the median endothelial cell loss was 29% (range 20-51%, P = 0.015), at 100 mmHg 54% (range 37-69%, P < 0.001), at 150 mmHg 75% (range 41-88%, P < 0.001), at 300 mmHg 91% (range 63-100%, P < 0.001) and at short high-pressure exposure 65% (range 49-82%, P < 0.001) in comparison with 20% (range 0-44%) at baseline. Significant neointimal proliferation occurred when a distension pressure of 50 mmHg was exceeded at 50 mmHg, median neointimal proliferation was 97 µm (range 60-380 µm, P = 0.176), at 100 mmHg 168 µm (range 100-600 µm, P = 0.001), at 150 mmHg 183 µm (range 160-440 µm, P < 0.001) at 300 mmHg 347 µm (range 190-590 µm, P < 0.001) and at short high-pressure exposure 130 µm (range 60-410 µm, P = 0.02) in comparison with 90 µm (range 60-170 µm) at baseline.CONCLUSIONS:
In vitro exposure of SVGs to low distension pressure ranges causes significant acute endothelial cell loss and crucial long-term damage, namely neointimal proliferation.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Presión
/
Vena Safena
/
Puente de Arteria Coronaria
/
Células Endoteliales
/
Neointima
Tipo de estudio:
Clinical_trials
/
Evaluation_studies
Límite:
Humans
Idioma:
En
Año:
2012
Tipo del documento:
Article