[Comparative study on the HPV infection rate of different esophageal squamous cell carcinoma in Anyang China].

OBJECTIVE: Comparative and statistical analysis the HPV infection rate between fresh tissue and Paraffin-embedded Specimens of esophageal squamous cell carcinoma,and comparative the testing results with others regions. METHODS: Extracted the total DNA from the novel fresh tissue and Paraffin-embedded Specimens; Detected the DNA by PCR with universal primer and Detected the HPV type with human papilloma virus nucleic acid amplification-based typing detection reagent kit (Hybribio); Compared the statistical result from the different specimens; analyzed the result between different region. RESULTS: HPV infection rate of fresh tissue is 82.6% with HPV16 (34.8%) and HPV18 (34.8%), and paraffin-embedded specimens is 78.2% with HPV16 (30.4%) and HPV18 (17.4%). CONCLUSION: The results provides the first evidence that there wasn't noticeable difference between HPV infection rate of the two specimens. So broader specimen source could be used for HPV testing.