The grapevine expression atlas reveals a deep transcriptome shift driving the entire plant into a maturation program.
Plant Cell
; 24(9): 3489-505, 2012 Sep.
Article
en En
| MEDLINE
| ID: mdl-22948079
ABSTRACT
We developed a genome-wide transcriptomic atlas of grapevine (Vitis vinifera) based on 54 samples representing green and woody tissues and organs at different developmental stages as well as specialized tissues such as pollen and senescent leaves. Together, these samples expressed â¼91% of the predicted grapevine genes. Pollen and senescent leaves had unique transcriptomes reflecting their specialized functions and physiological status. However, microarray and RNA-seq analysis grouped all the other samples into two major classes based on maturity rather than organ identity, namely, the vegetative/green and mature/woody categories. This division represents a fundamental transcriptomic reprogramming during the maturation process and was highlighted by three statistical approaches identifying the transcriptional relationships among samples (correlation analysis), putative biomarkers (O2PLS-DA approach), and sets of strongly and consistently expressed genes that define groups (topics) of similar samples (biclustering analysis). Gene coexpression analysis indicated that the mature/woody developmental program results from the reiterative coactivation of pathways that are largely inactive in vegetative/green tissues, often involving the coregulation of clusters of neighboring genes and global regulation based on codon preference. This global transcriptomic reprogramming during maturation has not been observed in herbaceous annual species and may be a defining characteristic of perennial woody plants.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Genes de Plantas
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Genoma de Planta
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Regulación de la Expresión Génica de las Plantas
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Vitis
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Transcriptoma
Tipo de estudio:
Prognostic_studies
Idioma:
En
Año:
2012
Tipo del documento:
Article